In vivo evaluation of [161Tb]Tb-crown-αMSH made with 161Tb purified from both methods displayed almost identical biodistribution of [161Tb]Tb-crown-αMSH in mice bearing B16-F10 tumours at 2 h post injection, demonstrating the 161Tb purified from small columns is interchangeable with HPIC purified 161Tb for such purposes. This evidence concerns the gene STAMBP and neoplasm.