(D) Representative pictures of VPS9D1-AS1-negative (+, C11) and VPS9D1-AS1-positive (G15, ++++) and multispectral fluorescence immunohistochemistry (mfIHC)-stained TGF-β, TGFBR1, and SMAD1/5/9 in the same CRC patients. This evidence concerns the gene SMAD1 and colorectal carcinoma.