We also performed KEGG pathway analysis and found the differentially expressed genes (DEGs) enriched in several cancer pathways such as Mitophagy, PI3K-Akt signaling pathway, Cytokine-cytokine receptor interaction, Rap1 signaling pathway, and focal adhesion, suggesting that a wide range of crucial cancer cell characteristics can be influenced by PUS1 through a complex regulatory network during tumor progression (Figure 3F). Here, PUS1 is linked to cancer.