Conversely, following procedure reported earlier to knockout of TSGs in lung cancer mouse model of Lsl-KrasG12D transgenic mice through CRISPR/Cas9 [28, 29], we intranasally delivered lentivirus targeting either Td-Tomato (serving as a control, K-sgTD) or ZNF24 (K-sgZNF24) into Lsl- KrasG12D mice (Additional file 4: Figure S2M, N). The gene discussed is ZNF24; the disease is lung cancer.