In addition, we analyzed the expression of these 4 genes in breast cancer tissues using data from Cancer Genome Atlas (TCGA) and draw box plots using GEPIA (http://gepia.cancer-pku.cn/index.html) [25], which showed that RASGRF2, AKR1B1 and CRMP1 were low expressed in breast Cancer tissues, while RHOF was high expressed in breast Cancer tissues (Fig 2B). This evidence concerns the gene RHOF and breast cancer.