Strikingly, hTRPV1N331K-expressing cells did not show Ca2+ elevation in response to the TRPV1 agonists, even under conditions of HE levels and at saturating concentrations of the agonists (100 μM capsaicin, 500 nM RTX, and 5 μM DkTx; Figure 2, B–F, and Supplemental Figure 3, A–C). This evidence concerns the gene TRPV1 and hereditary elliptocytosis.