CD40LG and syphilis: Testing these samples by DPP HIV 1/2, DPP HIV-Syphilis, STAT-PAK HIV 1/2, and Sure Check HIV 1/2, each employing SpA-gold nanoparticles, produced antibody-positive results in all four POCTs, demonstrating their ability to detect IgM responses developed prior to IgG-mediated seroconversion.