Ten different species of bacteria were isolated and identified, in which 93.65% of participants living with HIV presented with bacteria that is related to middle ear pathologies, while 6.4% had no ear infections. A total of 3.1% of children had middle ear pathologies and 0.3% of children showed no signs of ear infections. The prevalence rate of bacteria associated with otitis media was 93.4% and a lot of bacteria were resistant to antibiotics. Linear association between the prevalence of OAB with both duration of ART and CD4 counts were observed. This evidence concerns the gene CD4 and ear infection.