TNF and influenza: As the network analysis and molecular docking results showed that the TRAF6/MAPK14 axis might play the key pathogenic role in the treatment of influenza by CSTRG, we further examined the anti-inflammatory activity of CSTRG using LPS-stimulated RAW264.7 macrophages in vitro model, in which the stimulation of LPS can cause RAW264.7 differentiated into spindle-shaped M1-like macrophages accompanied by upregulation of TNF-α and followed by the release of inflammatory factors (27).