The collected tissues were prepared by slice preparation, and the results showed that the liver, spleen and kidney were congested 100 days after ALV-A rHB2015012 infection (Figures 10B,F,J), whereas the liver, spleen and kidney were congested after ALV-A rHB2015012 infection after A20 interference. The gene discussed is TNFAIP3; the disease is infection.