PFKM and cancer: A functional enrichment analysis of DEPs was performed using GO and KEGG, in which the HIF-1 signalling pathway, biosynthesis of secondary metabolites, central carbon metabolism in cancer, and glycolysis/gluconeogenesis extract had six important DEPs (phosphoglycerate kinase (PGK), hexokinases (HK), lactate dehydrogenase (LDH), phosphoglycerate Mutase (PGAM), phosphofructose kinase(pfkA) and pyruvate dehydrogenase kinase (PDK1) (Table 4).