SOX4 and Insulin resistance: We further found that overexpression of Sox4 in preadipocytes reduced the body weight of mice with HFD (Figure S1A), relieved glucose intolerance as well as insulin resistance (Figure S1B-C), and improved the heat production without obvious effect on food intake and locomotor activity (Figure S1D, E), which suggested that SOX4 may regulate thermogenesis in response to certain external stimuli.