• More oligoclonal compared to SP or DN CD8+ TILs.• Recognize and kill higher proportion of autologous tumor cells.• Higher frequency of granzyme B、4-1BB、Ki-67-positive cells.• PMA-responsive genes and mitochondrial genes Keep stable.• Polyfunctional T Cells that can Produce IL-21 and GM-CSF. Here, CSF2 is linked to neoplasm.