For TMB analysis, we found that the 15 most highly mutated genes were TP53, TTN, KMT2D, MUC16, ARID1A, KDM6A, PIK3CA, SYNE1, KMT2C, RYR2, HMCN1, RB1, FAT4, MACF1, and EP300 in BLCA. The gene discussed is MACF1; the disease is bladder transitional cell carcinoma.