For TMB analysis, we found that the 15 most highly mutated genes were TP53, TTN, KMT2D, MUC16, ARID1A, KDM6A, PIK3CA, SYNE1, KMT2C, RYR2, HMCN1, RB1, FAT4, MACF1, and EP300 in BLCA. Here, SYNE1 is linked to bladder transitional cell carcinoma.