Following this approach, Amanat et al.10 reported a burden of rare missense variants in patients with severe tinnitus in several genes including ANK2, AKAP9 and TSC2. Our results confirm that severe tinnitus is associated with hyperacusis, hearing loss and anxiety, according to the HADS and THI scores in both sexes, consistent with large epidemiological studies7,16. This evidence concerns the gene TSC2 and Tinnitus.