Currently, the main markers utilized for the detection of senescence include SA-β-gal, cell cycle-related p53, p16, p21, DNA damage-related γH2A.X, nuclear morphological marker Lamin B1, and the SASP component IL-6.11–13 Our present results indicate that the p21 and p53 expression was evidently raised in AKI induced by cisplatin with a decrease in phosphorylated Rb. The gene discussed is CDKN2A; the disease is acute kidney injury.