Collectively, these results revealed that mice beard xenograft tumors from 5-Fu-resistant, PTBP1-knockdown gastric cancer cells were more sensitive to 5-Fu treatment through inhibition of glycolysis, suggesting the combination of PTBP1 inhibition with 5-Fu treatments renders a synergistically anti-tumor effect against chemoresistant gastric tumors. Here, PTBP1 is linked to gastric cancer.