The role of TRPM2 in migration and invasion of neuroblastoma was studied further using SH-SY5Y cells depleted of TRPM2 with CRISPR technology (KO1-V, KO2-V), TRPM2 KO cells reconstituted with full length TRPM2 (KO1-M2, KO2-M2) or the TRPM2 Ca2+-impermeant mutant E960D (KO1-E960D, KO2-E960D), or scrambled control cells generated at the time of CRISPR KO (Scr1-V, Scr2-V) (Fig. 2)19. The gene discussed is RBMS1; the disease is neuroblastoma.