The most significant discovery was the best performance of isoAsp-related biomarkers (AUC = 0.92) in detection of mild cognitive impairment (MCI) compared with other current blood biomarkers (Aβ42/Aβ40 ratio, p-Tau181, neurofilament light chain (NfL) and glial fibrillary acidic protein (GFAP)). The gene discussed is NEFL; the disease is Cognitive impairment.