To clarify the benefits of CPSF6 inhibition in LUAD, we knocked down the expression of the CPSF6 gene in two human LUAD cell lines, A549 and NCI-H1299, by lentivirus-mediated RNA interference (RNAi) and evaluated the alterations in lung cancer proliferation and apoptosis. The gene discussed is CPSF6; the disease is lung carcinoma.