The results of PAS staining of intestinal sections at 1 week after challenge showed that intestinal villus width, goblet cell number and mucin 2 expression level of mice immunized with TsCRT+TsSP1.1, TsCRT or TsSP1.1 were remarkably lower than the empty pcDNA3.1 and PBS groups, indicating that oral vaccination of mice with TsCRT/TsSP1.1 clearly impeded larva intrusion of gut mucosa, relieved gut inflammation and alleviated the infection severity. Here, MUC2 is linked to infection.