To determine whether the kidney function is impaired in the Zeb2-cKO mice, we measured the blood urea nitrogen (BUN) and found that the 3-week-old Zeb2-cKO mice had nearly 4-fold increased BUN levels (100.6 ± 18.9 mg/dL in Zeb2-cKO mice) as compared with their wild-type littermates (26 ± 7.2 mg/dL), indicating impaired kidney function and kidney failure (Figure 2F and Table 1). Here, ZEB2 is linked to kidney failure.