BSCL2 and colorectal carcinoma: We found that human HCT116 diploid cells derived from colorectal carcinoma (Roschke et al., 2002), which we use exclusively for gene knockout analysis, expressed only the short form of Seipin, designated SeipinS, whereas human neuroblastoma-derived SH-SY5Y cells with trisomy 7 (Yusuf et al., 2013) expressed both SeipinS and the long form of Seipin, designated SeipinL, and further showed that SeipinS and SeipinL were both sensitive to digestion with endoglycosidase H (Endo H) (Figure 1B(a); note that the same amounts of total proteins [10 μg] in cell lysates were analyzed).