In our study, TCRαβ+ DNT cells secreting more granzyme A in HIV/TB co-infection than that in HIV infection and healthy controls, and TCRαβ+ DNT cells secreting more granzyme A in TB than that in healthy controls, suggesting that TCRαβ+ DNT cells might exhibit cytotoxic T cells-like function in TB no matter with or without HIV infection. Here, GZMA is linked to tuberculosis.