To further verify if rs2955626, rs684232 and rs461251 are directly involved in the regulation of VPS53, FAM57A and GEMIN4, we applied the CRISPR/Cas9 genome editing approach in PCa cell line V16A and obtained four independent mixed cell clones with partial depletion of each SNP enhancer region at 17p13.3. This evidence concerns the gene VPS53 and posterior cortical atrophy.