In order to evaluate the mammary-specific progression of carcinomas driven by LATS1/2 loss, we introduced Ad-K8-nlsCre into the mammary ducts of control and LATS1/2-floxed mice harboring an LSL-EYFP or LSL-tdTomato lineage trace33 using intraductal injections34 and followed these mice over time. Here, LATS1 is linked to carcinoma.