Furthermore, such prodrug nanoparticles could cause DNA damage to activate the STING pathway and stimulate a powerful immune response, which could then induce the maturation of DCs and tumor infiltration of CD8+ T cells in tumors, resulting in the elicitation of antitumor immunity in vivo and finally transforming “immune cold tumors” into “immune hot tumors” for combined chemotherapy and cancer immunotherapy. This evidence concerns the gene CD8A and cancer.