The brain sections from 6- and 24-month-old male AppNLGF, AppNL and WT mice were stained with antibodies against p-tau 217 and p-tau 231, which are CSF and plasma biomarkers of the preclinical stage of Alzheimer’s disease.21,23,24 These antibodies detected p-tau 217 and p-tau 231 signals in the cortex (Fig. 1A and B) and hippocampus (Supplementary Fig. 1A and B) of AppNLGF mice, while no clear signal was detected in the brains of either AppNL or WT mice (Fig. 1A and B). This evidence concerns the gene MAPT and early-onset autosomal dominant Alzheimer disease.