qPCR analysis and Western blot analysis showed that YTHDF1 expression was upregulated in ELK1-overexpressing prostate cancer cells (Figure 7B-D), and dual-luciferase reporter assays suggested that ELK1 transduction enhanced the transcriptional activity of the luciferase reporter flanked by the YTHDF1 promoter in both PC-3 and DU145 cells, indicating that ELK1 may transactivate YTHDF1 expression (Figure 7E). The gene discussed is YTHDF1; the disease is prostate carcinoma.