To determine whether miR-494-3p down-regulates BMAL1 expression by directly binding to the 3'UTR of BMAL1 in HCC cells, we subcloned wild-type or mutant miR-494-3p binding sequences from the BMAL1 3'UTR into luciferase reporter vectors (Figure S6A). Here, BMAL1 is linked to hepatocellular carcinoma.