To additionally confirm the suppressive role of BMAL1 in the growth and metastasis of HCC, we over-expressed BMAL1 by transfecting the BMAL1 expression vector or empty vector into MHCC97H and Hep3B cells with relatively low BMAL1 expression, as indicated in Figure 1F and 1G. This evidence concerns the gene BMAL1 and hepatocellular carcinoma.