SMAD4 and neoplasm: To increase the detection power and accuracy, we combined our samples and the TCGA/Tumor Portal cohort samples (a total of 200 ACGEJ samples) and found 11 significant driver genes (FDR q< 0.1): TP53, ARID1A, MUC6, SMAD4, PIK3CA, KRAS, PTEN, CDKN2A, MAP2K7, RNF43 and RHOA. (Figure 1A).