In contrast, upregulated pathways in early luminal tumors were more limited, but were noticeably more prevalent in later luminal tumors, including upregulation of MAPK, Notch, PI3K-AKT-mTOR, EGF, and TP53 pathways, and downregulation of HIPPO signaling, G-Protein Coupled Receptor Signaling, and Cell Cycle G1/S Checkpoint Regulation, all of which have been associated with the development and progression of human bladder cancer (30, 39, 40, 68–74). This evidence concerns the gene EGF and urinary bladder carcinoma.