Figure 3A shows a greater abundance of fibroblasts (p< 0.05), Treg (p< 0.001), and CD56 (p< 0.001) and a lower abundance of CD8 (p< 0.05) in cluster 2 chordomas, which was confirmed using immunohistochemistry (Figure 3E). The risk group had an identical result with the cluster group. However, the low-risk group had a greater abundance of fibroblasts (p< 0.01), Treg (p< 0.05), and CD56 (p< 0.01) and a lower abundance of CD8 (p< 0.05) when compared with those of the high-risk group (Figure 3B). The gene discussed is NCAM1; the disease is chordoma.