As shown in Figure 1F, higher OGT protein and O-GlcNAc levels were detected in both AR-positive (LNCaP, 22RV1) and AR-negative (PC3, DU145) PC cell lines, whereas OGA showed a lower expression, compared to those in human normal prostatic matrix immortalized cell wpmy-1. Here, OGT is linked to pachyonychia congenita.