The protein levels of TNF-α in the CSF (Figure 3C) were also significantly elevated in HF + Scr siRNA rats, along with increased mRNA expression of TNF-α (Figure 3D), TNFR1 (Figure 3E), and COX-2 (Figure 3G) in the PVN compared with SHAM + Scr siRNA rats. This evidence concerns the gene TNF and hydrops fetalis.