In view of the carcinogenic effect of the hub gene, was further verified by independent cohort from GEO and the results identified that the hub gene POLR2A was low expressed in virus-related HCC, and POL2B, RPL5, RPS6, and RPL23A were high expressed, which were consistent with the expression in the internal data sets. The gene discussed is RPL23A; the disease is hepatocellular carcinoma.