Since a large number of M2-type macrophages infiltrate HCC caused by HBV and HCV, and the activation of PI3K-Akt-mTOR pathway is related to the M2 polarization of macrophages (11, 24), Through literature review, it was found that 13 differential genes (25–36), including mTOR, PIK3CA, AKT2, and PIK3CB, involved in the M2-type polarization of macrophages, according to the genes related to M2-type polarization. This evidence concerns the gene PIK3CD and hepatocellular carcinoma.