Cytokine production levels in the pre-seasonal ASIT regimen had moderate to high correlation with allergic signs for IL-4 (r=0.52-0.56, P=0.013-0.009, vs. ear swelling test, lung pathology), IL-5 (r=0.63-0.85, P=0.035-0.002, vs. rhinitis, ear swelling test, lung pathology), IL-13 (r=0.68-0.78, P=0.029-0.001, vs. rhinitis, ear swelling test, or lung pathology), TNF-α (r=0.63, P=0.002, vs. ear swelling test), and highest negative correlation for IFN-γ and ratio of IFN-γ/IL-4 (r=-0.49-0.50, P=0.022-0.024, vs. rhinitis symptoms). This evidence concerns the gene TNF and rhinitis.