Although the long multi-epitope peptide plus lenalidomide and anti-PD1 vaccine showed enhanced specific lysis against GBM target cells through enhanced IFN-γ secreting, the cells expressing inflammatory molecules, such as IFN-γ, IL-10, TNF-α, in the re-stimulated splenocytes and re-stimulated lymph nodes were not identified in this study. This evidence concerns the gene PDCD1 and glioblastoma.