F2R and gastric cancer: Given the known associations between the seven signaling pathways identified from the IPA analysis of the microarray results and the PI3K/Akt signaling pathway, as well as literature reports of the associations between ncRuPAR, PAR-1, and PI3K/Akt signaling 12-14,17,18, the effect of ncRuPAR expression level on PAR-1 and PI3K/Akt signaling pathway was explored in HGC-27 and MGC-803 gastric cancer cell lines in vitro by real-time qPCR (Fig. 6A-B) and western blot analysis (Fig. 6C-D and Supplementary material 2).