Meanwhile, IGSF9 was strongly negatively correlated with immunostimulators C10 or f54 and strongly negatively correlated with immunosuppressants CD244, PDCD1, and TGFB1. This deepened the understanding of the signal pathway related to IGSF9 in NPC and confirmed that IGSF9 can be used as a suitable diagnostic and prognostic gene for the prognosis of NPC. Here, TGFB1 is linked to nasopharyngeal carcinoma.