Specifically, PF-00835231 suppressed SARS-CoV-2 Wuhan-Hu-1, Washington strain 1, Belgium/GHB-03021/2020 infection in A549, Calu-1, Vero E6, Vero E6-enACE2 (Vero E6 kidney cells enriched with ACE2), and Vero E6-EGFP (Vero E6 cells constitutively expressing EGFP) cells with EC50 values ranging from 24.7 nM to 88,900 nM in the absence of P-gp inhibitors (such as CP-100356 and elacridar). The gene discussed is ACE2; the disease is infection.