Furthermore, our histologic analysis (including Oil red O and HE stainings) demonstrated that Ad-PLZF treatment apparently stimulated the hepatosteatosis, including inducing massive deposit of large lipid droplets and ballooning degeneration of liver cells, which marked a process that leads to NAFLD and finally to non-alcoholic steatohepatitis (NASH) (Figure 1F). Here, ZBTB16 is linked to metabolic dysfunction-associated steatohepatitis.