Among the intersecting parts, we found that cell cycle checkpoints, mitotic G2-M phases, Rho GTPase activation, and GPCR ligand binding were significantly associated with PFDN1/2/3/4 alterations, and these pathways are also referred to the progression and tumorigenesis of HCC (Figures 5A–D). This evidence concerns the gene PFDN1 and hepatocellular carcinoma.