ACTA1 and colorectal carcinoma: Second, in CRC-LM adjacent to normal liver tissue, triple immunofluorescence (IF) staining of pan-cytokeratin (hepatocytes; Figure 2D, zone marked “a”), α-SMA (stellate cells/fibroblasts) and LTBP2 revealed a distinct enrichment of LTBP2+ cells in the portal regions of normal liver (Figure 2D, zone marked “b”).