We confirmed the expression of three signature genes specific to ECM-CAFs (LTBP2, C3, and POSTN) by RT-qPCR in relevant mesenchymal cells: a stellate cell line (LX2), fibroblasts (CCD18Co), and CAFs isolated from a CRC-LM sample (CRC-LM CAFs). This evidence concerns the gene LTBP2 and colorectal carcinoma.