To validate this hypothesis in liver-specific deletion of Hpcal1 and established the clinical relevance between HPCAL1 and key driver genes of human HCC, we take advantage of hydrodynamic tail-vein injections to generate a MYC/Trp53-/- liver cancer model in which oncogenic MYC can be genomically integrated, and that TP53 is deficient to recapitulate the features of HCC, as previously described 34. Here, TP53 is linked to hepatocellular carcinoma.