Supplement ATP or adding the antioxidant NAC robustly ameliorated TIMM44 silencing (by shTIMM44-seq1)-induced viability (CCK-8 OD) reduction and cell death (Trypan blue staining) in P1 glioma cells (Figure S3A and B), suggesting that mitochondrial dysfunction should be the primary cause of TIMM44-depeletion-induced cytotoxicity in glioma cells. The gene discussed is TIMM44; the disease is central nervous system cancer.