Similar to our results in P1 glioma cells, we found that TIMM44 shRNA decreased mitochondrial complex I activity (Figure S3C) and ATP contents (Figure S3D), while increasing the ROS levels (CellROX intensity increase, Figure S3E) in P2/ P3 primary glioma cells and established lines (A172 and U251). This evidence concerns the gene TIMM44 and central nervous system cancer.