DHFR and central nervous system cancer: TIMM44 shRNA (by shTIMM44-seq1) or KO robustly inhibited the import of Su9-DHFR fusion protein into the mitochondria of P1 glioma cells (Figure S4A), and about 60-70% reduction of mitochondrial protein import was achieved with TIMM44 silencing/KO (Figure S4A).