Moreover, consistent with the ability of SIAIS361034 and GDC-0449 against tumor growth, RT-qPCR analysis revealed that SIAIS316034 significantly inhibited the expression of Gli1 mRNA in tumor tissues, while no alteration of the Gli1 mRNA levels was observed in tumors exposed to GDC-0449 (Figure 6E). The gene discussed is GLI1; the disease is neoplasm.