GSDME and neoplasm: To verify this speculation, we screened tfRFP-B16 tumor cells with moderate or high expression of GSDME with green fluorescence protein (GFP) (Figure S9A) or without GSDME expression (Figure S9B), grouped as GSDME-GFPmidtfRFP-B16 cells, GSDME-GFPhitfRFP-B16 cells, and GSDME-/-tfRFP-B16 cells, respectively.