In the TCGA-LIHC cohort, the expression of DKC1, PUS1, PUS3, PUS7, PUS7L, PUSL1, RPUSD1, RPUSD2, RPUSD3, RPUSD4, TRUB1, and TRUB2 was upregulated in HCC tissues While there was no difference in the expression of PUS10 (Figure 2C). The gene discussed is RPUSD2; the disease is hepatocellular carcinoma.