In the LIRI-JP cohort, DKC1, PUS1, PUS7, PUS7L, PUSL1, RPUSD1, RPUSD2, RPUSD3, RPUSD4, TRUB1, and TRUB2 were upregulated in HCC tissues, and PUS10 was downregulated. Here, RPUSD4 is linked to hepatocellular carcinoma.