A cohort of 168 patients affected by calcific severe aortic stenosis was analyzed by targeted sequencing using a custom panel of 9 genes, selected among those more frequently mutated in CHIP, namely: DNMT3A, TET2, ASXL1, JAK2, NOTCH1, TP53, KDM6A, CBL, and NF1. This evidence concerns the gene NOTCH1 and aortic stenosis.